Heterozygosity in diploid eukaryotes often makes genetic studies cumbersome. Methods that produce viable homozygous diploid offspring directly from heterozygous females allow F1 mutagenized females to be screened directly for deleterious mutations in an accelerated forward genetic screen. Streisinger et al. The "early pressure" EP method blocks the meiosis II, which occurs shortly after fertilization. The EP method produces a high percentage of viable embryos that can develop to fertile adults of either sex.
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In Vitro Fertilization Procedure 1. Move to the fertilization station and remove a plunger from the water in the test tube. Remove a capillary tube from its container and insert the plunger into the end of the capillary with the green mark.